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1.
International Journal of Energy Economics and Policy ; 13(3):56-60, 2023.
Article in English | Scopus | ID: covidwho-20245430

ABSTRACT

Recent years have been times of crisis triggered by the Covid-19 pandemic and the Russian attack on Ukraine. Energy markets have seen dynamic increases in commodity prices, which have continued to this day. The prices of oil, whose rise fuels inflation are particularly important for the world economy. The paper presents an overview and per-prospects for the development of transport in Poland during the energy crisis and the Covid-19 pandemic. The analysis carried out on the example of Poland clearly shows that in the near term there is no chance to reduce fuel prices in Poland, which would result in a decrease in transport costs. It seems that the only chance for companies to reduce transport costs is to change their supply and inventory management strategies to take advantage of economies of scale and maintain high inventory levels. On the other hand, in world markets, oil prices will fall only if the war in Ukraine finishes. © 2023, Econjournals. All rights reserved.

2.
Water ; 15(11):2132, 2023.
Article in English | ProQuest Central | ID: covidwho-20245287

ABSTRACT

Wastewater surveillance has been widely used to track the prevalence of SARS-CoV-2 in communities. Although some studies have investigated the decay of SARS-CoV-2 RNA in wastewater, understanding about its fate during wastewater transport in real sewers is still limited. This study aims to assess the impact of sewer biofilms on the dynamics of SARS-CoV-2 RNA concentration in naturally contaminated real wastewater (raw influent wastewater without extra SARS-CoV-2 virus/gene seeding) using a simulated laboratory-scale sewer system. The results indicated that, with the sewer biofilms, a 90% concentration reduction of the SARS-CoV-2 RNA was observed within 2 h both in wastewater of gravity (GS, gravity-driven sewers) and rising main (RM, pressurized sewers) sewer reactors. In contrast, the 90% reduction time was 8–26 h in control reactors without biofilms. The concentration reduction of SARS-CoV-2 RNA in wastewater was significantly more in the presence of sewer biofilms. In addition, an accumulation of c.a. 260 and 110 genome copies/cm2 of the SARS-CoV-2 E gene was observed in the sewer biofilm samples from RM and GS reactors within 12 h, respectively. These results confirmed that the in-sewer concentration reduction of SARS-CoV-2 RNA in wastewater was likely caused by the partition to sewer biofilms. The need to investigate the in-sewer dynamic of SARS-CoV-2 RNA, such as the variation of RNA concentration in influent wastewater caused by biofilm attachment and detachment, was highlighted by the significantly enhanced reduction rate of SARS-CoV-2 RNA in wastewater of sewer biofilm reactors and the accumulation of SARS-CoV-2 RNA in sewer biofilms. Further research should be conducted to investigate the in-sewer transportation of SARS-CoV-2 and their RNA and evaluate the role of sewer biofilms in leading to underestimates of COVID-19 prevalence in communities.

3.
Proceedings - 2022 2nd International Symposium on Artificial Intelligence and its Application on Media, ISAIAM 2022 ; : 43-47, 2022.
Article in English | Scopus | ID: covidwho-20243436

ABSTRACT

With the upgrading and innovation of the logistics industry, the requirements for the level of transportation smart technologies continue to increase. The outbreak of the COVID-19 has further promoted the development of unmanned transportation machines. Aimed at the requirements of intelligent following and automatic obstacle avoidance of mobile robots in dynamic and complex environments, this paper uses machine vision to realize the visual perception function, and studies the real-time path planning of robots in complicated environment. And this paper proposes the Dijkstra-ant colony optimization (ACO) fusion algorithm, the environment model is established by the link viewable method, the Dijkstra algorithm plans the initial path. The introduction of immune operators improves the ant colony algorithm to optimize the initial path. Finally, the simulation experiment proves that the fusion algorithm has good reliability in a dynamic environment. © 2022 IEEE.

4.
CEUR Workshop Proceedings ; 3382, 2022.
Article in English | Scopus | ID: covidwho-20242636

ABSTRACT

The pandemic of the coronavirus disease 2019 has shown weakness and threats in various fields of human activity. In turn, the World Health Organization has recommended different preventive measures to decrease the spreading of coronavirus. Nonetheless, the world community ought to be ready for worldwide pandemics in the closest future. One of the most productive approaches to prevent spreading the virus is still using a face mask. This case has required staff who would verify visitors in public areas to wear masks. The aim of this paper was to identify persons remotely who wore masks or not, and also inform the personnel about the status through the message queuing telemetry transport as soon as possible using the edge computing paradigm. To solve this problem, we proposed to use the Raspberry Pi with a camera as an edge device, as well as the TensorFlow framework for pre-processing data at the edge. The offered system is developed as a system that could be introduced into the entrance of public areas. Experimental results have shown that the proposed approach was able to optimize network traffic and detect persons without masks. This study can be applied to various closed and public areas for monitoring situations. © 2022 Copyright for this paper by its authors. Use permitted under Creative Commons License Attribution 4.0 International (CC BY 4.0).

5.
Advances in African Economic, Social and Political Development ; : 151-187, 2023.
Article in English | Scopus | ID: covidwho-20242371

ABSTRACT

African aviation has witnessed steady growth pre-COVID and as result of increased demand for air travel, there is an urgent need to improve the air transport infrastructure. This chapter examines the underlining complexities and challenges that are undermining the African region's propensity to exploit its growth trajectory. The chapter explores multiple differences in regional airport infrastructure. Infrastructure is considered a key component of the investment climate, reducing costs of doing business and enabling people to access markets. In general, Africa, by every measure of infrastructure coverage, lags behind their peers in other parts of the developing world. Poor infrastructure of most African airports is seen as a principal reason why the region continues to struggle to fulfil its undoubtedly economic potential. These infrastructure problems can hardly be solved due to limited financial resources and will therefore consequently lead to retaining infrastructure problems. The chapter proposes a series of blueprint measures in order to galvanize Africa's growth potential within air transport development. This calls for speeding up privatization and allowing more private equity investments to support air infrastructure improvements. The most desired option to finance airport infrastructure would be the Public–Private Partnership (PP). However, on the local level, banks have relative weak capital coffers, which also limit access to infrastructure capital loans. Investors see some underlining risks in financing airport projects in Africa, namely uncertainty related to forecasts of passenger growth numbers. Other risks are embedded in currency markets, whereby most domestic airport infrastructure with project revenues is generated in local currencies, but servicing foreign debt and equity involves payment in foreign currency. The chapter finally examines the impact of COVID-19 on airport operations. From 2019 to 2021, airports were severely affected by the global pandemic causing massive loss of revenues for both airport operators and airlines. © 2023, The Author(s), under exclusive license to Springer Nature Switzerland AG.

6.
Revue Medicale Suisse ; 16(701):1450-1455, 2020.
Article in French | EMBASE | ID: covidwho-20242152

ABSTRACT

The Covid 19 pandemic remains a serious public health problem until effective drugs and/or vaccines are available. Can we explain why so many people remain asymptomatic but nevertheless highly contagious explaining the speed with which the pandemic has spread around the world? Can we explain why the acute respiratory distress syndrome (ARDS) appears late but can so quickly have a fatal outcome? In the lung, mucociliary clearance (CMC) and alveolar clearance (CA) depend on the transport of sodium through the plasma membrane of epithelial cells. This transport is mediated by a highly selective sodium channel (Epithelial Sodium Channel = ENaC) which could be a key element in the pulmonary pathophysiology of SARS-CoV-2 infection.Copyright © 2020 Editions Medecine et Hygiene. All rights reserved.

7.
Nieren- und Hochdruckkrankheiten ; 52(4):134-135, 2023.
Article in English | EMBASE | ID: covidwho-20241899

ABSTRACT

Objective: COVID-19 has emerged as a significant global health crisis causing devastating effects on world population accounting for over 6 million deaths worldwide. Although acute RTI is the prevalent cause of morbidity, kidney outcomes centered on a spectrum of AKI have evolved over the course of the pandemic. Especially the emerging variants have posed a daunting challenge to the scientific communities, prompting an urging requirement for global contributions in understanding the viral dynamics. In addition to canonical genes, several subgroup- specific accessory genes are located between the S and E genes of coronaviruses regarding which little is known. Previous studies have shown that accessory proteins (aps) in viruses function as viroporins that regulate viral infection, propagation and egress [1]. In this study we attempted to characterize the function of aps of coronavirus variants as ion channels. Furthermore, we also probed the interaction of ap4 with the host system. Method(s): Serial passaging (selection pressure), growth kinetics, confocal imaging, genome sequence analysis and proteomics were performed in Huh-7, MRC5 cells and/or human monocyte derived macrophages. Potassium uptake assay was performed in a Saccharo myces cerevisiae strain, which lacks the potassium transporters trk1 and trk2. Ion conductivity experiments were performed in Xenopus laevis oocytes using Two Electrode Voltage Clamp (TEVC) method. Result(s): Serial passaging demonstrated the acquisition of several frameshift mutations in ORF4 resulting in C-terminally truncated protein versions (ap4 and ap4a) and indicate a strong selection pressure against retaining a complete ORF4 in vitro. Growth kinetics in primary cells illustrated a reduction of viral titers when the full-length ap4 was expressed compared to the C-terminally truncated protein ap4a. Confocal imaging showed that ap4 and ap4a are not exclusively located in a single cellular compartment. Potassium uptake assay in yeast and TEVC analyses in Xenopus oocytes showed that ap4 and ap4a act as a weak K+ selective ion channel. In addition, accessory proteins of other virus variants also elicited microampere range of currents. Conclusion(s): Our study provides the first evidence that ap4 and other accessory proteins of coronavirus variants act as viroporins. Future studies are aimed at demonstrating the role of ap4 during the viral life cycle by modulating ion homeostasis of host cell in vivo (interacting proteins obtained from proteomic studies) and thereby serve as a tool for potential drug target.

8.
Chinese Journal of Food Hygiene ; 34(6):1282-1285, 2022.
Article in Chinese | CAB Abstracts | ID: covidwho-20241582

ABSTRACT

To summarize thepractice and experience of targeted food hygiene security measures in a major field activity of the army in order to provide references for diverse tasks. Considering the characteristics of heavy activity, field operations and the influence of COVID-19, a series of support measures related to food hygiene surveillance were strengthened. The first measure was review of recipes, health management and training of employees, procurement and storage of raw materials, warehouse management, processing and manufacturing management, disinfection of tableware, as well as food sample retention. Secondly, the control points that probably cause spread of COVID-19 in the phase of food service industry were analyzed, then relevant supervision and guidance were carried out from the aspects of employees and diners, foods of cold chain logistics, environment and emergency response plan. Finally, in order to assure the safety of food processing and crowd-gathered diet in the field, the following measures were guided to adopted including selecting the site of cooking and dining properly, cleaning the environment, making dishes using pure water and semi-manufactured foodstuff, keeping food sanitation in the course of transport and dinning, as well as supervising the robot machines for cooking automatically. The main experiences were listed as follows: promoting food safety awareness of the principal and the employees of the canteen, enhancing legal enforcement capacity and technical capacity of health supervisors, focusing on new risks related to food safety as well as reinforcing the management of health supervisors and employees in the field.

9.
Safety and Risk of Pharmacotherapy ; 10(4):365-380, 2022.
Article in Russian | EMBASE | ID: covidwho-20241278

ABSTRACT

Timely, effective, and safe antiviral therapy in COVID-19 patients reduces complications, disability and mortality rates. The greatest concern with remdesivir is the risk of drug-induced liver injury, including in patients whose liver function is compromised by COVID-19. The aim of the study was to investigate the efficacy and safety of remdesivir in patients with confirmed SARSCoV-2 infection who had been admitted to an infectious diseases hospital in the Volgograd region in March 2022. Material(s) and Method(s): the authors carried out an open, non-randomised, single-arm study using medical records of 234 patients who had been diagnosed with "U07.1 COVID-19, virus identified" and prescribed remdesivir upon admission. The effectiveness of therapy was evaluated using two criteria: the need for oxygen supplementation or ventilatory support, or mortality. The authors conducted the evaluation on days 7, 14, and 28 using the six-point ordinal severity scale by Y. Wang et al. The safety of therapy was assessed on the basis of complaints and changes in laboratory findings. Result(s): for the patients prescribed remdesivir at admission, the 7-day mortality rate was 3.0%, the 14-day mortality rate was 5.6%, and the 28-day mortality rate was 7.3%. With the exception of a patient with myocardial infarction, all the patients who had been hospitalised with mild COVID-19 and prescribed remdesivir did not require oxygen therapy and/or transfer to intensive care and were discharged following recovery. The patients with moderate to severe COVID-19 had the 14-day mortality rate of 6.4% and the 28-day mortality rate of 8.6%. 17 patients (7.2%) discontinued remdesivir prematurely for various reasons, including adverse drug reactions. Remdesivir therapy of 5-10 days was associated with an increase in ALT activity by 2.7 +/- 0.8 times in 15.9% of patients with mild COVID-19, by 3.8 +/- 1.8 times in 20.4% of patients with moderately severe COVID-19, and by 4.8 +/- 2.7 times in 24% (12/50) of patients with severe COVID-19. In two patients (0.9%), the increase exceeded 10-fold the upper limit of normal. Conclusion(s): the obtained results support recommending remdesivir to patients with mild, moderate and severe COVID-19, including those with moderately elevated baseline activity of hepatic transaminases.Copyright © NEICON ISP LLC. All rights reserved.

10.
New Nationalisms and China's Belt and Road Initiative: Exploring the Transnational Public Domain ; : 245-258, 2022.
Article in English | Scopus | ID: covidwho-20240647

ABSTRACT

Kazakhstan, being in the centre of the Eurasian continent, is a landlocked country that shares a border both with China and Russia. Since its independence in 1991, Kazakhstan has attempted to take advantage of its geographical location, by becoming a bridge between Europe and China. To achieve this goal, Kazakhstan has actively participated in the construction of the Silk Road Economic Belt (SREB) and joined the Asian Infrastructure Investment Bank (AIIB) as one of the founding countries. These activities have brought Kazakhstan not only the opportunity to become a transport and logistics hub but also to reduce its dependence on Russian infrastructure. In the light of the current war in Ukraine, the transportation of goods through the territory of Kazakhstan becomes more crucial to China as it can bypass Russia. Though the SREB has enabled Kazakhstan to export more resources and become more independent from Russian infrastructure, same time, China might become a threat to Kazakhstan's economic independence, as it will be more connected to the Chinese infrastructure. This chapter also shows that during the COVID-19 pandemic, China's economic policy in Kazakhstan is evolving. China now spends less on large infrastructure projects and focuses more on Kazakhstan's raw materials. This study aims to take a comprehensive look at Kazakhstan's relationship with China, as well as the implications of the SREB for Kazakhstan and its transport capacity. © The Editor(s) (if applicable) and The Author(s), under exclusive licence to Springer Nature Switzerland AG 2022.

11.
33rd Congress of the International Council of the Aeronautical Sciences, ICAS 2022 ; 9:6493-6501, 2022.
Article in English | Scopus | ID: covidwho-20240545

ABSTRACT

This work is an analysis of the implications of the potential new regulatory policies being recently proposed in the European Union, in particular the mandatory blending of SAF (on top of the already existing CORSIA or ETS), to address aviation emissions from a technical, operational and economic perspective. As a continuation of previous work from the Department of Aerospace Systems, Air Transport and Airports from the ETSIAE (Universidad Politécnica de Madrid), the air traffic structure of the European Union in 2019 has been analysed based on publicly available data from EUROCONTROL and EUROSTAT. The output has been used as the reference scenario for the implementation of the mandatory blending of SAF, expected to take over at the beginning of 2025, since it is expected that by then, air traffic will reach the pre-COVID levels. The results show that all the policy options considered so far have uneven impact among the different stakeholders and that before deciding going forward with any of the presented options, extra work needs to be done to overcome the different challenges that would potentially arise. © 2022 ICAS. All Rights Reserved.

12.
Transportation Research Procedia ; 69:902-909, 2023.
Article in English | Scopus | ID: covidwho-20240528

ABSTRACT

Further to a first benchmark study covering new mobility behaviours and their impact on the road infrastructure, carried out by the European Union Road Federation (ERF), the Confederation of International Contractor's Associations (CICA), the French Federation of Public Works (FNTP), the European Construction Industry federation (FIEC) and Routes de France in 2019-2020, the same group published a second study in September 2021. The objective of that second study was to give a picture of the impact of the crisis caused by the pandemic on the mobility and transport sectors in 11 European countries (Belgium, Croatia, Czech Republic, France, Germany, Italy, Netherlands, Poland, Spain, Sweden, United Kingdom) The approach was to compare the evolution of mobility before and after the emergence of the health crisis. In addition, it would analyse the way in which European countries have adapted their support for transport infrastructures, further to an analysis of National Recovery and Resilience Plans, based on the European Recovery Plan ("Next Generation EU"). Beyond the main trends observed and their impact on mobility patterns and habits, the group also made recommendations on the role of road in the global mobility framework and the necessary adaptation of the road transport infrastructure. © 2023 The Authors. Published by ELSEVIER B.V.

13.
Advances in Transportation Studies ; 60:141-158, 2023.
Article in English | Academic Search Complete | ID: covidwho-20240044

ABSTRACT

This paper contains an investigation of the COVID-19 impacts on freight flows and the handling of uncertainty in freight forecasting models, based on data from Greece. It collects and analyses, over a 7-year period before and during the pandemic, data for freight transport operations and some related factors in order to macroscopically examine any statistically significant changes in their values over time. This period wasjudged necessary in order to establish the pattern of fluctuations in the relevant data during the non-pandemic years and thus make the visual comparison with the previous period and the years during the pandemic, more clear. First, the paper tests the impact of the pandemic as expressed by the number of daily COVID-19 cases on freight flow variables in order to find the dynamic behavior of these variables and trace their reactions over time. This analysis is made by using the Vector Autoregressive Model (VAR). By implementing VAR modelling, we analyzed the dynamic relationship between freight transport volumes and other factors such as GDP, the industrial production index, exporting transactions and the number of coronavirus cases. The main result of the model analysis and the employment of impulse response functions revealed that the unexpected shock of COVID has a negative reaction to the economy and the freight transport volumes and a rather shortterm limited duration disruption effect on the growth of exports as well as on the industrial production index, of approximately eight months. Secondly, the paper discusses how, unpredicted events like the pandemic, influence the uncertainty inherent in freight transport modelling and formulates a novel freight modelling framework procedure based on scenario building, regular monitoring and data updates on a permanent basis. [ FROM AUTHOR] Copyright of Advances in Transportation Studies is the property of Advances in Transportation Studies and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full . (Copyright applies to all s.)

14.
Proceedings of the Institution of Civil Engineers: Municipal Engineer ; 2023.
Article in English | Scopus | ID: covidwho-20239972

ABSTRACT

For the past years, the world has been facing one of the worst pandemics of modern times. The COVID-19 outbreak joined a long list of infectious diseases that turned pandemic, and it will most likely leave scars and change how we live, plan, and manage the urban space and its infrastructures. Many fields of science were called into action to mitigate the impacts of this pandemic, including spatial and transport planning. Given the large number of articles recently published in these research areas, it is time to carry out an overview of the knowledge produced, synthesising, systematising, and critically analysing it. This article aims to review how the urban layout, accessibility and mobility influence the spread of a virus in an urban environment and what solutions exist or have been proposed to create a more effective and less intrusive response to pandemics. This review is split into two avenues of research: spatial planning and transport planning, including the direct and indirect impact on the environment and sustainability. © 2023 ICE Publishing: All rights reserved.

15.
Iranian Journal of Pediatrics ; 33(3) (no pagination), 2023.
Article in English | EMBASE | ID: covidwho-20239636

ABSTRACT

Introduction: The people worldwide have been affected by severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) infection since its appearance in December, 2019. Kawasaki disease-like hyperinflammatory shock associated with SARS-CoV-2 infection in previously healthy children has been reported in the literature, which is now referred to as a multisystem inflammatory syndrome in children (MIS-C). Some aspects of MIS-C are similar to those of Kawasaki disease, toxic shock syndrome, secondary hemophagocytic syndrome, and macrophage activation syndrome. Case Presentation: This study reported an 11-year-old boy with MIS-C presented with periorbital and peripheral edema, abdominal pain, elevated liver enzymes, severe right pleural effusion, moderate ascites, and severe failure of right and left ventricles. Conclusion(s): Due to the increasing number of reported cases of critically ill patients afflicted with MIS-C and its life-threatening complications, it was recommended that further studies should be carried out in order to provide screening tests for myocardial dysfunction. Adopting a multidisciplinary approach was found inevitable.Copyright © 2023, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

16.
LOGI - Scientific Journal on Transport and Logistics ; 14(1):181-191, 2023.
Article in English | Scopus | ID: covidwho-20239412

ABSTRACT

The unexpected emergence of the viral disease COVID-19 has caused many changes in all areas of life. People suffered from the fear of contracting the disease, which resulted in the decrease in the use of public passenger transport services. People's uncertainty and frequently changing measures with a lack of information caused a shift of passengers from public passenger transport to individual transport. The analysis identified important criteria that passengers considered very important during the COVID-19 pandemic in relation to public transport. If these criteria are not met, passengers will not be willing to use public passenger transport and the use of individual transport will continue to grow. To ensure passenger satisfaction, new innovative software solutions have been analysed and then implemented into one specific solution. The proposed solution can meet the requirements of passengers, facilitate their travel using public passenger transport and provide them with regular information related to viral illnesses and important information and will ensure their satisfaction. © 2023 Jaroslav Mašek et al.

17.
LOGI - Scientific Journal on Transport and Logistics ; 14(1):169-180, 2023.
Article in English | Scopus | ID: covidwho-20239147

ABSTRACT

The effects of the COVID-19-related crisis in society can be seen not only in long-distance passenger transport but also in the urban transportation services in the territory. During the pandemic, the travelling public tended to choose private motor vehicles, bicycles, or walking as their mode of transport to work or other activities. This fact was also the subject of our research based on the analysis of data on public passenger rail transport in the individual regions of the Slovak Republic and based on data analysis performed, also in urban transport in the city of Žilina. Mobility in the city was investigated and analysed based on detailed monitoring of the use of the means of transport on a specific trolleybus line No. 67, where a hybrid trolleybus Škoda 26TrA Solaris was deployed. It was found out that the impact of the pandemic and the adoption of restrictive measures to prevent the spread of the disease significantly affected this trolleybus line, where, depending on the extent of the measures taken, the occupancy of the trolleybus fluctuated by up to 60 % in specific months compared to the standard period of use of this means of transport. © 2023 Peter Majerčák et al.

18.
LOGI - Scientific Journal on Transport and Logistics ; 14(1):158-168, 2023.
Article in English | Scopus | ID: covidwho-20239146

ABSTRACT

In this scientific paper, the authors address the issue of mobility and the spread of COVID-19, as well as the impact of the COVID-19 pandemic and changes in the mobility of the population of the Czech Republic and Slovakia in 2020. The goal of the study is to determine whether the increase in the number of positive cases of COVID-19 and the associated increase in deaths influences changes in various categories of population mobility at the regional level in selected countries. The aim of the work is to empirically assess the impact of the first year of the pandemic on the mobility of the population. To achieve this, data on population mobility obtained from Google Statistics, together with information on the increase in the number of positive cases and the number of deaths caused by COVID-19 from both countries, were combined. The paper interprets and evaluates the results achieved, providing a picture of the course of the pandemic in the territories of the Czech Republic and Slovakia. © 2023 Jozef Majerčák et al.

19.
American Journal of Clinical Pathology, suppl 1 ; 158:S140-S141, 2022.
Article in English | ProQuest Central | ID: covidwho-20238466

ABSTRACT

Introduction/Objective The public health emergency of the COVID-19 pandemic emphasized the crucial role of medical laboratory professionals and scientists in molecular diagnostics laboratories to ensure success in infection control strategies. The demand for laboratory testing using nucleic acid amplification tests to detect SARS-CoV-2 RNA imposed strains in laboratory supplies. Here, we explored an alternative cost-effective solution that will simplify the pre-PCR steps by using a simple heating method to release viral RNA. Methods/Case Report Samples tested using the reference automated extraction method were used:100 samples identified as positive for SARS-CoV-2 RNA and 500 samples tested negative for SARS-CoV-2 RNA were used for the study and sorted with equal distribution according to Ct values of (a) <20, (b) 20–30, and (c) >30.100 ul from swab preserved in Universal Transport Medium was treated with 30 μg of proteinase K, and another set was tested without proteinase K pre-treatment. All samples with or without proteinase K were diluted to minimize PCR inhibitors. The thermal shock protocol was set at (98°C, 5 minutes;4°C, 2 minutes) and screened for purity. Performance and method verification studies were performed. Internal extraction, positive template, and no template controls were markers used for testing quality. The experimental study was performed by qualified testing personnel and all under the same experimental conditions. Results (if a Case Study enter NA) The Ct values from the thermal shock RNA release were compared to the automated extraction method and statistically analyzed.The criteria for acceptability for validation of this new RNA extraction proceeding were set to 100% concordance compared to the commercial kit using an automated extraction. PCR efficiency was at 98% and a slope of -3.3. Within run precision of 2% and limits of detection from 200 to 20,000 copies/uL The method 100% (50/50) concordance on samples previously identified as negative by automated methods and identified 86% (86/100) with a mean difference of 3 Ct. Conclusion Our findings suggest that the thermal shock treatment of nasopharyngeal swabs in viral transport media can successfully extract viral nucleic acid for nucleic acid amplification and is a reasonable alternative for chemical extraction methods when molecular diagnostic laboratories persistently encounter supply chain issues.

20.
LOGI - Scientific Journal on Transport and Logistics ; 14(1):146-157, 2023.
Article in English | Scopus | ID: covidwho-20238087

ABSTRACT

The COVID-19 pandemic and the anti-pandemic measures taken have significantly affected the activities of the society and the associated need for mobility, as well as the transport behaviour of inhabitants. The goal of this research is to assess the impact of the COVID-19 pandemic on the change in the demand of residents for suburban bus transport (SBT) services in the regions of Slovakia. Due to the impact of the pandemic of COVID-19, there was a decrease in the number of passengers transported by SBT as well as a decrease in the supply of bus services offered in all the regions under study. The decrease in the number of passengers in the pandemic year was caused not only by COVID-19 and the anti-pandemic measures, but also by the reduction in the supply of SBT. The research confirmed the relation between the reduction in the offer of SBT and the decrease in passenger demand. © 2023 Vladimír Konečný et al.

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